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Promega pf3a wg flexi vector
Pf3a Wg Flexi Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf3a+wg+flexi+vector/pf3a/bio_rxiv__2024__09__03__611025-262-31-37
Average 90 stars, based on 1 article reviews
pf3a wg flexi vector - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species-Dependent Signaling during the Initial Response to Salt Stress in Rice
Article Snippet: .. To obtain protein for EMSA assays, the SERF1 CDS was subcloned into the pF3A WG Flexi vector (Promega). .. For in vitro expression, 3.5 μg of plasmid DNA was added to the TNT SP6 High Yield Wheat Germ Mastermix containing 1 μL FluoroTec Green Lys (Promega).

Article Title: Salt-Related MYB1 Coordinates Abscisic Acid Biosynthesis and Signaling during Salt Stress in Arabidopsis
Article Snippet: .. EMSA and ChIP - qPCR To obtain protein for EMSA assays, the SRM1 CDS was subcloned into the pF3A WG Flexi vector (Promega). ..

Article Title: Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development
Article Snippet: .. In short, the CDS of KUA1 was cloned into the pF3A WG Flexi vector (Promega), which contains translation enhancer sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, that facilitates in vitro expression of proteins in wheat germ extracts. .. For the in vitro expression, 5.0 μg of plasmid DNA was added to TNT SP6 High Yield Wheat Germ Mastermix (Promega) containing 1 μl FluoroTec Green Lys (Promega).

Article Title: MULTIPASS, a rice R2R3-type MYB transcription factor, regulates adaptive growth by integrating multiple hormonal pathways.
Article Snippet: .. To this end, the OsMPS CDS was cloned into the pF3A WG Flexi vector (Promega, Mannheim, Germany) and translated with the TNT® SP6 High Yield Wheat Germ Mastermix containing 1 μL FluoroTec Green Lys (Promega). .. EMSAs were performed with Cy5-labelled probes (Table S10) using the LightShift® Chemiluminescent Assay Kit (Pierce, Rockford, USA) according to the manufacturer's instruction.

Chromatin Immunoprecipitation:

Article Title: Salt-Related MYB1 Coordinates Abscisic Acid Biosynthesis and Signaling during Salt Stress in Arabidopsis
Article Snippet: .. EMSA and ChIP - qPCR To obtain protein for EMSA assays, the SRM1 CDS was subcloned into the pF3A WG Flexi vector (Promega). ..

Real-time Polymerase Chain Reaction:

Article Title: Salt-Related MYB1 Coordinates Abscisic Acid Biosynthesis and Signaling during Salt Stress in Arabidopsis
Article Snippet: .. EMSA and ChIP - qPCR To obtain protein for EMSA assays, the SRM1 CDS was subcloned into the pF3A WG Flexi vector (Promega). ..

other:

Article Title: USP37 prevents premature disassembly of stressed replisomes by TRAIP
Article Snippet: The wild-type Xenopus HA-USP37 open reading frame (ORF) was cloned by introducing the respective gBlocks (IDT) using NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the AsiSI (NEB) and EcoRI-HF (NEB) double-cut pF3A WG (BYDV) Flexi vector (Promega).

Clone Assay:

Article Title: Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development
Article Snippet: .. In short, the CDS of KUA1 was cloned into the pF3A WG Flexi vector (Promega), which contains translation enhancer sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, that facilitates in vitro expression of proteins in wheat germ extracts. .. For the in vitro expression, 5.0 μg of plasmid DNA was added to TNT SP6 High Yield Wheat Germ Mastermix (Promega) containing 1 μl FluoroTec Green Lys (Promega).

Article Title: MULTIPASS, a rice R2R3-type MYB transcription factor, regulates adaptive growth by integrating multiple hormonal pathways.
Article Snippet: .. To this end, the OsMPS CDS was cloned into the pF3A WG Flexi vector (Promega, Mannheim, Germany) and translated with the TNT® SP6 High Yield Wheat Germ Mastermix containing 1 μL FluoroTec Green Lys (Promega). .. EMSAs were performed with Cy5-labelled probes (Table S10) using the LightShift® Chemiluminescent Assay Kit (Pierce, Rockford, USA) according to the manufacturer's instruction.

Virus:

Article Title: Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development
Article Snippet: .. In short, the CDS of KUA1 was cloned into the pF3A WG Flexi vector (Promega), which contains translation enhancer sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, that facilitates in vitro expression of proteins in wheat germ extracts. .. For the in vitro expression, 5.0 μg of plasmid DNA was added to TNT SP6 High Yield Wheat Germ Mastermix (Promega) containing 1 μl FluoroTec Green Lys (Promega).

In Vitro:

Article Title: Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development
Article Snippet: .. In short, the CDS of KUA1 was cloned into the pF3A WG Flexi vector (Promega), which contains translation enhancer sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, that facilitates in vitro expression of proteins in wheat germ extracts. .. For the in vitro expression, 5.0 μg of plasmid DNA was added to TNT SP6 High Yield Wheat Germ Mastermix (Promega) containing 1 μl FluoroTec Green Lys (Promega).

Expressing:

Article Title: Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development
Article Snippet: .. In short, the CDS of KUA1 was cloned into the pF3A WG Flexi vector (Promega), which contains translation enhancer sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, that facilitates in vitro expression of proteins in wheat germ extracts. .. For the in vitro expression, 5.0 μg of plasmid DNA was added to TNT SP6 High Yield Wheat Germ Mastermix (Promega) containing 1 μl FluoroTec Green Lys (Promega).



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